Yong Guo,Yang Wang,Yinqin Liu,Haitao Wang,Chun Guo,Xizheng Zhang,Chaoyong Bei.[J].Chin J Traumatol,2015,18(3):150-156. [doi]
Effect of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro
  
DOI：
KeyWord: Mechanical loadingOsteoblastsMesenchymal stem cellsRAW264.7 cellsOsteogenesisOsteoclastogenesis
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Author NameAffiliation
Yong Guo College of Biotechnology, Guilin Medical University 
Yang Wang College of Biotechnology, Guilin Medical University 
Yinqin Liu College of Biotechnology, Guilin Medical University 
Haitao Wang College of Biotechnology, Guilin Medical University 
Chun Guo Department of Medicine, Luohe Medical College 
Xizheng Zhang Institute of Medical Equipment, Academy of Military Medical Sciences 
Chaoyong Bei Department of Orthopedic, Affiliated Hospital of Guilin Medical University 
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Abstract:
      Purpose: To investigate the influence of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro. Methods: Primary osteoblasts, bone marrow-derived mesenchymal stem cells (BMSCs, cultured in osteoinductive medium) and RAW264.7 cells cultured in osteoclast inductive medium were all subjected to a 1000 mstrain (ms) at 1 Hz cyclic mechanical stretch for 30 min (twice a day). Results: After mechanical stimulation, the alkaline phosphatase (ALP) activity, osteocalcin protein level of the osteoblasts and BMSCs were all enhanced, and the mRNA levels of ALP and collagen type I increased. Additionally, extracellular-deposited calcium of both osteoblasts and BMSCs increased. At the same time, the activity of secreted tartrate-resistant acid phosphatase, the number of tartrate-resistant acid phosphatase-positive multinucleated cells, matrix metalloproteinase-9 protein levels of RAW264.7 cells and the extracellular calcium solvency all decreased. Conclusion: The results demonstrated that 1000 ms cyclic mechanical loading enhanced osteoblasts activity, promoted osteobla
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