Zhang Qin,Huang Chen,Meng Bin,Tang Tiansi,Yang Huilin.[J].Chin J Traumatol,2014,17(4):193-197. [doi]
Changes in autophagy proteins in a rat model of spinal cord injury
  
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KeyWord: Spinal cord injuries  Autophagy  LC3 protein, rat  Cathepsin D
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Author NameAffiliation
Zhang Qin Department of Orthopaedics, Yuncheng Central Hospital, Yuncheng 044000, Shanxi Province, China 
Huang Chen Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215006, China 
Meng Bin Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215007, China 
Tang Tiansi Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215008, China 
Yang Huilin Department of Orthopaedics, First Affiliated Hospital of Soochow University, Suzhou 215009, China 
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Abstract:
      Objective: Autophagy is involved in several neurodegenerative diseases and recently its role in acute brain injury has won increasing interest. Spinal cord injuries (SCIs) often lead to permanent neurological deficit. Therefore, in this study, we examined the profiles of autophagy-linked proteins (MAP-LC3) after SCI to investigate whether the expression of autophagy contributes to neurological deficit after SCI. Methods: Adult female Sprague-Dawley rats were used and randomly divided into control and SCI groups. All the rates received laminectomy at T8-T10 level. Those in the SCI group received additional exposure of the dorsal surface of the spinal cord, followed by a weightdrop injury. Thereafter we investigated the expression levels of MAP-LC3, beclin-1, Cathepsin D and the beclin-1-binding protein bcl-2 by western blot analysis at 12 h, 24 h, 3 d, 7 d, 21 d and 28 d. One-way ANOVA with Tukey post hoc test was used to compare data between groups. Results: We observed significant increase in the level of LC3 (LC3-II/LC3-I) at 3 d and 7 d after SCI when compared with the sham group. While the level of beclin-1 and ratio of beclin-1/bcl-2 was found to have increased from 12 h to 24 h after injury. Cathepsin D expression was also elevated at 7 d (P<0.01). Conclusion: Based on the above mentioned data, we proposed that autophagy plays a role in the manifestation of cell injury following SCI.
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