Jin Peng,Zhu Lielie,Zhang Jiasheng,Xie Songling,Pan Da,Wen Hao,Meng Weiyang,Lin Luyang,Chen Daqing.[J].Chin J Traumatol,2014,17(3):125-129. [doi]
A correlation study of the expression of resistin and glycometabolism in muscle tissue after traumatic brain injury in rats
  
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KeyWord: Brain injuries  Resistin  Insulin resistance  Blood glucose  Insulin sensitivity
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Author NameAffiliation
Jin Peng Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, China 
Zhu Lielie Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325028, China 
Zhang Jiasheng Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325029, China 
Xie Songling Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325030, China 
Pan Da Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325031, China 
Wen Hao Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325032, China 
Meng Weiyang Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325033, China 
Lin Luyang Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325034, China 
Chen Daqing Emergency Center, Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325035, China 
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Abstract:
      Objective: To investigate the expression pattern of resistin (RSTN) in skeletal muscle tissue and its influence on glycometabolism in rats with traumatic brain injury (TBI). Methods: Seventy-eight SD rats were randomly divided into traumatic group (n=36), RSTN group (n=36) and sham operation group (n=6). Fluid percussion TBI model was developed in traumatic and RSTN groups and the latter received additional 1 mg RSTN antibody treatment for each rat. At respectively 12 h, 24 h, 72 h, 1 w, 2 w, and 4 w after operation, venous blood was collected and the right hind leg skeletal muscle tissue was sampled. We used real-time PCR to determine mRNA expression of RSTN in skeletal muscles, western blot to determine RSTN protein expression and ELISA to assess serum insulin as well as fasting blood glucose (FBG) levels. Calculation of the quantitative insulin sensitivity check index (Q value) was also conducted. The above mentioned indicators and their correction were statistically analyzed. Results: Compared with sham operation group, the RSTN expression in the skeletal muscle as well as serum insulin and FBG levels revealed significant elevation (P<0.05), and reduced Q value (P<0.05) in traumatic group. Single factor linear correlation analysis showed a significant negative correlation between RSTN expression and Q values (P<0.001) in traumatic group. Conclusion: The expression of RSTN has been greatly increased in the muscular tissue of TBI rats and it was closely related to the index of glycometabolism. RSTN may play an important role in the process of insulin resistance after TBI.
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