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| SUN Hai-biao,CHEN Jun-chang.[J].Chin J Traumatol,2013,16(1):158-162. [doi] |
| Prevention of bone loss by injection of insulin-like growth factor-1 after sciatic neurectomy in rats |
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| DOI： |
| KeyWord: Osteoporosis Sciatic nerve Osteoblasts Insulin-like growth factor 1 |
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| Abstract: |
| Objective: Injection of insulin-like growth factor-1 (IGF-1) can prevent bone loss in sciatic
nerve transaction rats. We try to investigate the action mechanism of IGF-1 on bone formation.
Methods: Atotal of40 adult male Spragne-Dawleyrats were divided into two groups (experimental group and control group) with 20 animals in each. Sciatic neurectomy was performed to model disuse osteoporosis in all rats. IGF-1 was administered in experimental group with the dose of 100 µg/kg per day for 3 days. Meanwhile, the rats in control group were treated with saline. Bone mineral density was measured by dual-energy X-ray absorptiometry 4 and 6 weeks after neurectomy respectively. Expression of Osterix and Runx2 was determined by reverse transcription-polymerase chain reaction (RT-PCR) assay.
Results: There was a significant increase in the bone mineral density of experimental group compared with control group. There was a significant decrease in the level of receptor activator of nuclear factor- κB-ligand but an increase in the level of osteoprotegerin 4 and 6 weeks after neurectomy in the experimental group compared with control one. The expression of Osterix and Runx2 was up-regulated in the bone marrow ofexperimentalgroupcomparedwith control group.
Conclusion: IGF-1 can increase bone formation by stimulation of osteoblast number and activity, and reduce bone resorption by restriction of differentiation of osteoclast, suggesting that IGF-1 may improve the therapeutic efficacy for disuse osteoporosis. |
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